Pick Lab Protocols

Oncopeltus fasciatus (milkweed bug) Protocols
​Oncopeltus embryo injection
​Oncopeltus fixation & in situ
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Jadera haematoloma (red-shouldered bug) Protocols
Jadera embryo fixation & in situ

Drosophila melanogaster Protocols 
Drosophila embryo collection notes
​Drosophila embryo fixation
General colorimetric in situ
​Double in situ - colorimetric
Single fluorescent in situ
TSA Doubles

Tribolium casteneum (flour beetle) Protocols
Tribolium rearing
​Tribolium DNA extraction
​Tribolium embryo fixation
​Tribolium in situ - colorimetric
Tribolium double in situ - colorimetric
​Tribolium pupal injections (pRNAi)
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​Dermestes maculatus  (hide beetle) Protocols
Dermestes diet
Dermestes embryo fixation
Dermestes embryo in situ hybridization
JoVE paper on Dermestes rearing & RNAi

Murgantia histrionica (harlequin bug) Protocols
Murgantia embryo fixation
Murgantia embryo in situ

BMSB Protocols (coming soon)

Callosobruchus maculatus Protocols
Mung beetle rearing, embryo collection, fixation
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Anopheles stephensi (Asian malaria mosquito) Protocols
Anopheles rearing
Anopheles embryo fixation

​Vanessa cardui (painted lady butterfly) Protocols
​Vanessa embryo fixation & in situ
Vanessa embryo injection

CRISPR diagnostics - any species
​Heteroduplex assay